Ant and Dec decided enough was enough and called out Amir for his behaviour toward Iain in tonight's episode.
The comments came as the boxer was once again making cruel remarks toward the radio host.
Dec stated: "There's loads of celebrities down here who will have done stuff they don't want to be reminded of."
He continued to jibe: "Remember the guy who shouted, 'I'm A Celebrity…Get Me Out Of Here!' in the first trial?"
Ant and Dec call out Amir Khan on I'm A Celeb
"Amir," Ant replied.
Ant continued to list off several of Amir's failings, noting how hypocritical he had been toward Iain.
Fans of the show took to Twitter to express their delight in the hosts' comments.
I'm A Celeb: Ant and Dec take swipe at Amir Khan
Amir Kkan in the I'm A Celeb Bush Telegraph
One viewer wrote: "Ant and Dec slagging Amir off is the best thing to happen yet this series #ImACeleb."
"Still find it funny that Ant and Dec don’t like Amir either, they’ve literally just spoken for the whole nation #ImACeleb," said another.
Amir told Iain he had "f****d up a lot of things" when he entered the jungle a few days later than the original contestants.
Iain confessed in the Bush Telegraph that Amir's comments made him feel "uncomfortable."